Water is a critical component of all living cells.
Interestingly, tissue membranes show a great degree of water permeability.
Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water.
Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein “aquaporin.” Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues.
Aquaporin-0 or MIP26 (major intrinsic protein 26kD) and Aquaporin-1 (purified from red cells), or CHIP-28 (channel forming integral protein, 28kD; 268aa; gene locus 7p14) have been the foundation of the growing family of aquaporins.
The lens specific Aquaporin-0 represents up to 80% of total lens membrane protein.
Defects in MIP26 are a cause of autosomal dominant cataract.
The cataract Fraser mutation (CAT-FR or Shriveled) is a transposon-induced splicing error that substitutes a long terminal repeat sequence for the c-terminus of MIP.
The lens opacity mutation (LOP) is an AA substitution that inhibits targeting of MIP to the cell membrane.
Human Aquaporin-0 is a 263aa transmembrane protein belonging to the MIP family.
Aquaporin families of proteins are predicted to contain six transmembrane domains.
The N- and C-termini are predicted to be cytoplasmic.
Applications:Suitable for use in ELISA, Immunohistochemistry and Immunoflourescence.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilutions:ELISA: 0.5-1ug/ml; Coat plates with A3000-07M: Aquaporin 2, Rat, Control Peptide at 1ug/ml Immunohistochemistry: 2-10ug/ml in paraformaldehyde fixed tissue sections
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.1% BSA.
Specificity：Recognizes rat Aquaporin 2 (AQP-CD/WCH-CD).
Isotype：IgG
Calc Applications Abbrev：E IF IHC
Calc Crossreactivity：Rt
Immunogen：Synthetic peptide corresponding to 15aa within the C-terminus of rat AQP2, conjugated to KLH. Cellular Localization: Cytoplasmic. Peptide Sequence Homology: mouse, 100%; human and sheep, 93%.
