84-1028-73　Aquaporin 0 (AQP0, MIP26, Major Intrinsic Protein) 100ug　A2990

特徴

• Water is a critical component of all living cells.
• Interestingly, tissue membranes show a great degree of water permeability.
• Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water.
• Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporins".
• Over the last decade, genes for several members of aquaporin family (AQP0, AQP1-15) have been cloned, expressed, and their distribution studied in many tissues.
• The individual members of aquaporin family have identical predicted secondary structures with up to 6 highly conserved hydrophobic membrane spanning domains (about 18-25aa each) and two conserved NPA motifs.
• However, N/C-terminal regions of AQPs are only ~20% conserved.
• AQP0 or MIP26 (major intrinsic protein 26kD), and Aquaporin-1 (AQP1, purified from red cells) also called CHIP-28 (channel forming integral protein, 28kD; 26aa; gene locus 7p14) has been the foundation of the growing family of aquaporin.
• The lens specific AQP0 represents ~80% of total lens membrane protein.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilutions:ELISA: 1:100,000; Use 50-100ng control peptide/wellOptimal dilutions to be determined by researcher.
• Control Peptide:A2995-14: Aquaporin 0, Human, Control Peptide (AQP0, MIP26)
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.