Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver.
Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells.
ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents.
The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2.
Defects in apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000 Optimal dilutions to be determined by the researcher.
Positive Control: Liver tissue
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1ml
Host：goat
Source Antibody：human
Grade：Serum
Purity：Serum.
Form：Supplied as a liquid in TBS, pH 7.4, 0.09% sodium azide.
Specificity：Recognizes human Apo E. Species Crossreactivity: primate and mouse. Monospecific for Apolipoprotein E by IEP when tested against pooled human plasma and twice concentrated pooled human serum. Reacts with Apo E isoforms E2, E3 and E4 by Western blot.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mk Mo
Immunogen：Recombinant protein corresponding to human Apolipoprotein E.
