84-1028-67　Anion Exchanger 1 (AE1) 50ug　A2295-13A

特徴

• Anion exchangers (AE) are membranes proteins involved in the regulation of intracellular pH, cell volume regulation as well as in transepithelial acid/base transport.
• AE proteins are sodium-independent exchangers that mediates one-for-one exchange of extracellular Cl- for intracellular HCO3- ions resulting in intracellular acidification.
• AE proteins are encoded by a family of at least three related genes (AE1, AE2 and AE3).
• Numerous alternatively spliced isoforms of each AE gene are expressed in various tissues.
• AE proteins are exemplified by a large N-terminal cytoplasmic domain (~40-75kD) that provides binding sites for cytoskeleton protein, glycolytic enzymes and hemoglobin.
• The N-terminal cytoplasmic domains of AE2 are AE3 are more closely related than AE1.
• In fact, AE1 N-terminus is 300aa shorter than both the AE2 and AE3.
• The C-terminal TM domain (~55kD) is highly conserved (~70% identity) among various AE, spans the lipid bilayer 12-14 times.
• It is able to mediate anion exchange by itself.
• AE1 protein (also know as band 3) is the most abundant protein in red cell membrane.
• AE1 gene has been cloned and characterized from a variety of species including mouse, rat, human and chicken.
• Applications:Suitable for use in Western Blot and ELISA.
• Other applications have not been tested.
• Recommended Dilutions:Western Blot: 1-10ug/ml (ECL.) AE1 has a molecular weight of ~90-100kD.
• It aggregates easily forming dimers and multimers.
• It may also be proteolytically processed to smaller fragments of 60, 40 and 20kD
• ELISA: 1-10ug/ml.
• Using 1ug/ml A2295-13B4 control peptide per well.
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.