Amyloid Precursor Protein (APP) is an integral membrane protein with a large ectodomain, a transmembrane domain, and a short cytoplasmic tail.
APP is proteolytically cleaved by b-secretase (BACE) to generate the APP N-terminal fragment (sbAPP) with Mr100kD and a C-terminal fragment (C99) with Mr12kD.
The smaller 12kD fragment can be further cleaved by g-secretase, an enzyme activity recently attributed to presenilin-2.
This second cleavage produces the insoluble b-amyloid (Ab), a peptide of ~4kD.
The accumulation of b-amyloid in intracellular neurofibrillary tangles and extracellular plaques is observed in the brains of Alzheimer’s disease patients and Downs’s Syndrome patients.
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:250-1:1000 Immunoprecipitation: Need to reduce and alkylate disulfide bonds.
Optimal dilutions to be determined by the researcher
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in PBS, 0.01% azide.
Specificity：Recognizes the immature APP, a protein with Mr95kD and also the APP holoprotein, a posttranslationally modified protein with Mr100-120kD. Specific to aa99-126. Species crossreactivity: Monkey, mouse. Does not react with bovine.
