84-1028-65　Amyloid Precursor Protein, CT (APP, Amyloid beta (A4) Precursor Protein) 100ul　A2275-71B

特徴

• Amyloid Precursor Protein (APP) is an integral membrane protein with a large ectodomain, a transmembrane domain, and a short cytoplasmic tail.
• APP is proteolytically cleaved by b-secretase (BACE) to generate the APP N-terminal fragment (sbAPP) with Mr100kD and a C-terminal fragment (C99) with Mr12kD.
• The smaller 12kD fragment can be further cleaved by g-secretase, an enzyme activity recently attributed to presenilin-2.
• This second cleavage produces the insoluble b-amyloid (Ab), a peptide of ~4kD.
• The accumulation of b-amyloid in intracellular neurofibrillary tangles and extracellular plaques is observed in the brains of Alzheimer’s disease patients and Downs’s Syndrome patients.
• Applications:Suitable for use in Immunohistochemistry, Immunocytochemistry, Western Blot and Immunoprecipitation.
• Other applications have not been tested.
• Recommended Dilutions:Immunohistochemistry: 1:100Western Blot: 1:500-1:2000.
• The low abundance of full length APP in untreated cells means that poor response is seen with Western Blot of whole cell lysates.
• We recommend that membrane fractions be prepared to increase the loading of APP as well as remove potential interferences from soluable proteins.
• NTera2, Cos7 and Hela cell membrane preparations are positive by Western Blots.
• Immunoprecipitation: 1:100-1:400Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.