Minimum glutaraldehyde: 0.1%; works on paraffin embedded tissue (fixed with glutaraldehyde) but preferably epoxy embedded specifically developed for post-embedding protocols.
Living, isolated goldfish retinas were maintained for 10 minutes in an oxygenated physiological solution that preserves normal neuronal activity under constant lighting.
The medium also contained 10mM agmatine.
After the incubation period retinas were fixed in a conventional 2.5% glutaraldehyde buffer and processed for immunohistochemistry using 250nm sections probed with a gold-labeled secondary antibody.
The treated retinas were exposed to 125M kainate during the incubation.
Kainate opens both AMPA and kainate selective ionotropic channels through which agmatine can enter cells
Immunocytochemistry: On cells with glutamate-gated ion channels.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：2000Tests
Host：rabbit
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in PBS, 1% goat serum, 0.05% thimerosal.
Specificity：Recognizes Agmatine. Does not crossreact with arginine, glutamate or other amino acids. Is expected to recognize agmatine in mose vertebrate species.
