84-1028-53　IkB beta, CT 100ul　I2500-09

特徴

• NFkB exists in the cytoplasm as a complex with a 38kDa inhibitor protein, IkB (MAD-3).
• Activation of NFkB by different stimuli, including TNFa and PMA, requires the dissociation of the inhibitor protein.
• Dissociation is regulated by the phosphorylation of IkB on serine residues 32 and 36 which targets IkB for ubiquinitation and proteasome mediated degradation.
• The NFkB complex then translocates to the nucleus and activates transcription of responsive genes.
• Application(s):Suitable for Immunoprecipitation, Western Blot, ELISA.
• Recommended Dilution:ELISA: 1:1000-1:5000Western Blot: 1:500-1000 (Peroxidase conjugated goat anti-rabbit IgG (H&L).
• Anti-IkB beta is suitable for the detection by immunoblot of human, mouse and rat IkB beta.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
• Freeze at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.