84-1028-52　STAT 1, phosphorylated (Tyr701) (Signal Transducer and Activator of Transcription 1) 100ul　S7969-08

特徴

• STAT proteins (Signal Transduction and Activators of Transcription) are latent cytoplasmic transcription factors that have the dual function of signal transduction and activation of transcription.
• STATs are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus.
• The N-terminal region is highly homologous among the STAT proteins and surrounds a completely conserved arginine residue.
• STATs are a part of the JAK-STAT signaling pathway – a major pathway of the immune system.
• All cytokines transduce critical signals through this pathway.
• STAT 1, is activated by a number of different ligands, including IFNalpha, IFNgamma, EGF, PDGF and IL-6.
• Phosphorylation of tyrosine 701 is required for STAT 1 dissociation from IFNGR1, homodimerization, and nuclear translocation.
• Tyrosine 701 phosphorylation impairment results in loss of STAT 1 functions.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:500-1:2000 detects phosphorylated STAT1 in RIPA lysates from CNTF-treated SK-N-MC cells.
• 20ug of non-treated or CNTF-treated SK-N-MC cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with S7969-08 (1:1000).
• Proteins were visualized using an IgG (HRP) goat anti-rabbit secondary antibody and a chemiluminescence detection system.
• Control:Mouse 3T3-L1 adipocytes stimulated with leukemia inhibitory factor (LIF)
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.