84-1028-43　［取扱停止］DcR1 (Decoy Receptors for TRAIL, ED2, TRAIL-R3, TRID, LIT) 100ug　D1499-01

特徴

• Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors.
• TRAIL/Apo2L is a new member of the TNF family and induces apoptosis of a variety of tumor cell lines.
• DR4 and DR5 are the recently identified functional receptors for TRAIL.
• Two decoy receptors for TRAIL have been identified and designated DcR1/TRID/TRAIL-R3/LIT and DcR2/TRAIL-R4/TRUNDD.
• DcR1 has extracellular TRAIL-binding domain, but lacks intracellular signaling domain.
• It is a glycophospholipid-anchored cell surface protein.
• DcR1 transcripts are expressed in many normal human tissues, but not in most cancer cell lines.
• Overexpression of DcR1 did not induce apoptosis, but attenuated TRAIL-induced apoptosis.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.5-1ug/ml
• Optimal dilutions to be determined by the researcher.
• Positive Control:HeLa cell lysate
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
• Freeze at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.