84-1028-42　ASC (TMS1, Target of Methylation-induced Silencing) 100ug　A3598

特徴

• Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases.
• CARD containing cell death regulators include RAIDD, RICK, BCL10, Apaf-1, ARC, caspase-9, and caspase-2.
• A novel CARD domain containing protein was recently identified in human and mouse and designated ASC and TMS1 (1–3).
• Ectopic expression of ASC/TMS1 induced apoptosis through activation of caspase-9 and inhibited the survival of human breast cancer cells (3,4).
• Overexpression of ASC/TMS1 induced DNA fragmentation (4).
• ASC/TMS1 is expressed in a variety of human and mouse tissues (1, 2)
• Applications:Suitable for use in ELISA, Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blotting: 1ug/ml.
• Detects an ~25kD band
• Optimal dilutions to be determined by the researcher.
• Positive Control: HL60 whole cell lysate
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
• Freeze at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.