Ang II plays an important role in regulating natriuretic peptide gene expression during cardiac overload.
ANP and BNP gene expression in the rat heart is modulated by the adrenal factors during Ang II-stimulated hemodynamic stress and the AT1 receptor antagonism in NO-deficient hypertension.
Moreover, ventricular BNP gene expression in Ang II-induced hypertension in vivo is controlled by post-transcriptional mechanisms and GATA elements.
Applications:Suitable for use in Immunohistochemistry, Immunocytochemistry and Immunofluorescence.
Other applications not tested.
Recommended Dilutions:Immunofluorescence: 1:250-1:16,000Immunohistochemistry: Frozen and paraffinPAP, ABC: 1:250-1:16,000LAB-SA: 1:250-1:16,000Optimal dilution determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder. No stabilizing proteins and no preservatives added. Reconstitute with 50ul sterile ddH2O.
Specificity：Recognizes human Angiotensin II. Crossreactivity (RIA): Val5-Angiotensin II (human), Angiotensin III (human): 100%; Renin Substrate: 0.9%; Angiotensin I (human): 0.5%; a-ANP (human), Arg8-Vasopressin, Sar1, Ile8 Angiotensin II: 0%.
Isotype：IgG
Calc Applications Abbrev：IC IF IHC
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa25-32, H-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-OH of the human Angiotensin precursor.
