Certain serine/threonine protein kinases, such as ASK1, RIP, DAP, and ZIP kinases, are mediators of apoptosis.
Receptor interacting proteins including RIP and RIP2/RICK mediate apoptosis induced by TNFR1 and Fas, two prototype members in the death receptor family.
A novel member in the RIP kinase family was recently identified and designated RIP3 (1–3).
RIP3 contains N-terminal kinase domain but, unlike RIP or RIP2, lacks the C-terminal death or CARD domain.
RIP3 binds to RIP and TNFR1, mediates TNFR1 induced apoptosis, and attenuates RIP and TNFR1 induced NF-kB activation.
Overexpression of RIP3 induces apoptosis and NF-kB activation.
The messenger RNA of RIP3 is expressed in a subset of adult tissues (2,3).
Applications:Suitable for use in ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/ml.
A band at ~57kD can be detected
Immunocytochemistry/Immunofluorescence: 20ug/mlImmunohistochemistry: 5ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:3T3 cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Immunoaffinity chromatography purified IgG
Form：Supplied as a liquid in PBS containing 0.02% sodium azide.
Specificity：Recognizes mouse RIP3. Species crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：E IC IF IHC WB
Calc Crossreactivity：Mo Rt
Immunogen：Raised against a peptide (AQFGRGRGWQPFHK) corresponding to amino acids 473 to 486 of murine RIP3 (3).
