A novel mammalian CED-4 homologous was recently identified and cloned in mouse (1) and human (2) and designated FLASH (for FLICE-associated huge protein).
FLASH is involved in Fas induced apoptosis.
It is recruited to Fas after the receptor cross-linking.
Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis.
FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8 (1).
There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity (1,2).
FLASH is widely expressed.
Applications:Suitable for use in ELISA, Western Blotting.
Optimal dilutions to be determined by the researcher.
Positive Control: HeLa whole cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Immunoaffinity chromatography purified IgG
Form：Supplied as a liquid, 100ug in 200ul of PBS, 0.02% sodium azide.
Specificity：Recognizes human FLASH. An approximate 220kD band can be detected.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Raised against a synthetic peptide (SERFQQLMKLFEKSKC) corresponding to amino acids 1253 to 1268 of human origin, which differ from those of mouse by one amino acid (1,2).
