84-1028-37　［取扱停止］BACE2, Asp1 (CT) (Beta-site APP Cleaving Enzyme, Aspartyl Protease 2, DRAP, Down Region Aspartic Protease, Memapsin) 100ug　B0003-01

特徴

• Accumulation of the amyloid-b (Ab) plaque in the cerebral cortex is a critical event in the pathogenesis of Alzheimer’s disease.
• Ab peptide is generated by proteolytic cleavage of the b-amyloid protein precursor (APP) at b-and g-sites by proteases.
• The long-sought b-secretase was recently identified by several groups independently and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2) (1–4).
• A BACE homolog was recently cloned and designated BACE2, Asp1, DRAP (for Down region aspartic protease), and memapsin 1 (4–9).
• BACE2 also cleaves APP at b-site and at a different site within Ab (8).
• BACE2 locates on chromosome 21q22.3, the so-called ‘Down critical region’, suggesting that BACE2 and Ab may also contribute to the pathogenesis of Down syndrome (6,7)
• Applications:Suitable for use in ELISA, Western Blotting.
• Other applications not tested.
• Recommended Dilution:Western Blotting: 0.5-1ug/ml.
• Optimal dilutions to be determined by the researcher.
• Positive Control: Human heart tissue lysate can be used as positive control.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
• Freeze at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.