Chromatin condensation and nuclear fragmentation (CCNF) is the hallmark of apoptosis.
CCNF is triggered by the activation of members of caspase family, caspase activated DNase (CAD/DFF40), and several novel proteins including AIF and CIDE (1).
A new inducer of chromatin condensation was recently identified and designated Acinus (for apoptotic chromatin condensation inducer in the nucleus).
Acinus is cleaved by caspase-3 and an additional unknown protease generating a small active peptide p17, which causes chromatin condensation in vitro when it is added to purified nuclei.
Acinus also induces apoptotic chromatin condensation in cells.
Acinus is ubiquitously expressed.
Three different spliced forms of Acinus have been identified in human and mouse and designated AcinusL, AcinusS and AcinusS’ (2).
Applications:Suitable for use in ELISA, Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blotting: 0.25 to 0.5ug/ml
Optimal dilutions to be determined by the researcher.
Positive Control: K562 cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Immunoaffinity chromatography purified IgG
Form：Supplied as a liquid, 100ug in 200ul of PBS, 0.02% sodium azide.
Specificity：Detects human Acinus. Species crossreactivity: Mouse.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Rabbit anti-Acinus (CT) polyclonal antibody was raised against a peptide (SRSTPVRDRGGRR) corresponding to amino acids 1329 to 1341 of human AcinusL, 602 to 614 of human AcinusS’, or 571 to 583 of human AcinusS, which are identical to those of mouse Acinus (2).
