Lat is an adapter protein expressed in lymphoid cells, which is a substrate for ZAP70 and Syk protein kinases.
Phosphorylated LAT associates with GRB2, PLC-gamma1 and P85 PI 3-K.
Expression of a mutant LAT protein missing tyrosine phosphorylation sites leads to inhibition of T-cell activation, suggesting that the protein is an important signal transducer in T-cells
Applications:Suitable for use in Western Blot.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 0.5-2ug/ml detects phospho-LAT (Tyr191) in RIPA lysates from Jurkat cells stimulated with C305.
Jurkat cells were incubated without or with, a monoclonal antibody that binds Jurkat T-cell receptors.
Cell lysates were prepared, resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-LAT (Tyr191) (2ug/ml), or anti- LAT (1ug/ml).
Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
Specificity：Recognizes human phospho-LAT (Tyr191) (MW: 36kD)Species Crossreactivity: Mouse and human.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide (SREpYVNVSPEQQ-C) corresponding to aa188-195 of human LAT and aa192-203 of mouse LAT (KLH conjugated).
