84-1028-27　Platelet Derived Growth Factor Receptor alpha (PDGFRa) 200ul　P4258-26

特徴

• Two types of PDGF receptors have been identified: PDGF Receptor alpha (PDGFRa) and PDGF Receptor beta (PDGFRb).
• The PDGF Receptor alpha, a 170kD protein, binds all three isoforms of PDGF with high affinity whereas the PDGF Receptor beta, a 190kD protein, appears to bind only the PDGF BB homodimer with high affinity.
• Both receptors are transmembranous, ligand activated protein tyrosine kinases, which phosphorylate a number of important signal transduction proteins, which are bound with differential affinities via SH2 domains.
• The response of any given cell to PDGF will depend on the types of receptors displayed on the surface and isoforms of PDGF present in the extracellular environment
• Applications:Suitable for use in Western Blot and Immunoprecipitation.
• Other applications have not been tested.
• Recommended Dilutions:Western Blot: 1:500-1:1000 dilution detected PDGF Receptor Type A, a doublet (150/170kD) in 3T3/NIH cell lysate.
• 3T3/NIH cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with P4258-26 (1:500).
• Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system
• Immunoprecipitation: 5-10ul immunoprecipitates Platelet Derived Growth Factor Receptor alpha from 500ug of 3T3/NIH RIPA lysate
• Optimal dilutions to be determined by the researcher.
• Recommended Secondary Antibodies:I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRbI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRbI1904-46Q: IgG, H&L (HRP) Pab Gt xRb
• Storage and Stability:May be stored at 4°C.
• For long-term storage, aliquot and store at 4°C.
• Do not freeze.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Further dilutions can be made in assay buffer.