Angiopoietin-1 is an angiogenic secreted protein that interacts with endothelial specific Tie-2 receptor.
It is primarily expressed in developing endothelial cells.
During embryonic development, Ang-1 binds and induces tyrosine phosphorylation of Tie-2.
Ang-1 may play a critical role in heart development.
A homolog of Ang-1, termed Angiopoietin-2 (mouse and human Ang-2, 496 AA; ~85% identity) has recently been identified.
It may act as an antagonist for Ang-1 and Tie-2.
Ang-1 and Ang-2 have ~60% sequence homology.
It is prominently expressed in tissues that are in involved in vascular remodeling (ovary, placenta, and uterus).
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000-1:100,000 (50-100ng of control peptide/well)Western Blot: 1:1000-1:5000 (MW:~55kD)Optimal dilutions to be determined by the researcher.
Control Peptide (ELISA):A2293-12
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Serum
Purity：Neat serum
Form：Supplied as a lyophilized powder, 0.05% sodium azide. Reconstitute with 100ul sterile ddH2O or 40-50% glycerol.
Specificity：Recognizes mouse Ang-2. Immunogenic peptide has no significant sequence homology with Ang-1, Ang-3, Ang-4, or ARPs. Species Sequence Homology: rat:100%; porcine: 85%, human:71%; chicken: 55%.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Mo
Immunogen：Synthetic 20 amino acid peptide sequence near the N-terminal of mouse Ang-2 (KLH coupled).
