84-1027-91　Arachis hypogaea (PNA, Peanut Agglutinin) (Texas Red) 1ml　A3305-39

特徴

• Lectins are derived from the extracts of plants, animals, viruses and microorganisms and are known to agglutinate red blood cells.
• These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms.
• Lectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose
• Arachis hypogaea (PNA, Peanut Agglutinin), 110kD, is composed of four identical subunits of ~27kD each, is galactose-specific, with strong anti-T-antigen activity.
• T-antigen") is present in many glycoconjugates such as M and N blood groups, gangliosides, and many other soluble and membrane-associated glycoproteins and glycolipids.
• PNA does not agglutinate normal human erythrocytes, but strongly agglutinates neuraminidase-treated red blood cells. The presence of Ca++ can enhance the binding of PNA to receptors.
• Asialo GM1 ganglioside is also thought to be a major cell surface receptor for PNA.
• PNA is useful in normal and tumor tissue determination for transitional mucosa malignancies.
• Applications:Suitable for use in Immunofluorescence, Immunocytochemistry and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Optimal dilutions to be determined by the researcher.
• Carbohydrate Specificity: Terminal b-Galactose
• Inhibitory Carbohydrate: Lactose > b-Galactose
• Activity: ≤1ug/ml will agglutinate human erythrocytes neuraminidase treatment of the cells.
• Texas Red/Protein Ratio (OD595/OD280): 2.4
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Texas Red® conjugates are sensitive to light.