84-1027-89　Arachis hypogaea (PNA, Peanut Agglutinin) (FITC) 1ml　A3305-37

特徴

• Lectins are derived from the extracts of plants, animals, viruses and microorganisms and are known to agglutinate red blood cells.
• These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms.
• Lectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose.
• Arachis hypogaea (PNA, Peanut Agglutinin), 110kD, is composed of four identical subunits of approximately 27kD each, is galactose-specific, with strong anti-T-antigen activity.
• T-antigen") is present in many glycoconjugates such as M and N blood groups, gangliosides, and many other soluble and membrane-associated glycoproteins and glycolipids.
• PNA does not agglutinate normal human erythrocytes, but strongly agglutinates neuraminidase-treated red blood cells. The presence of Ca++ can enhance the binding of PNA to receptors.
• Asialo GM1 ganglioside is also thought to be a major cell surface receptor for PNA.
• PNA is useful in normal and tumor tissue determination for transitional mucosa malignancies.
• Applications:Suitable for use in Immunodiffusion, Immunohistochemistry, Immunocytochemistry and IEP.
• Other applications not tested.
• Recommended Dilutions:Immunohistochemistry: 20-100ug/mlImmunocytochemistry: 100ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.