Ricin is a protein toxin that is readily produced from castor beans (Lectin Ricinus communis) which inhibits protein synthesis.
Red blood cells agglutinate due to the presence of RCA (Ricinus communis agglutinin), a potent cytotoxin but a weak hemagglutinin.
Ricin and RCA are synthesized in the endosperm cells of maturing seeds, and are stored in an organelle called the "protein body", a vacuolar compartment.
When the mature seed germinates, the toxins are destroyed by hydrolysis within a few days.
Ricin is not catalytically active until it is proteolytically cleaved by an endopeptidase within the protein bodies.
This splits the polypeptide into the A chain and the B chain still linked by a single disulfide bond.
Since ricin is inactive until then, the plant avoids poisoning its own ribosomes in case some proricin accidentally passes into the cytosol during synthesis and transport.
The ricin A portion of the heterodimer is the enzyme that binds and depurinates a specific adenine of the 28S rRNA.
The adenine ring of the ribosome becomes sandwiched between two tyrosine rings in the catalytic cleft of the enzyme and is hydrolyzed by the enzyme’s N-glycosidase action.
The target adenine is a specific RNA sequence that contains the unusual tetranucleotide loop, GAGA.
Ricin is more active against animal than plant ribosomes, and intact bacterial ribosomes are generally not susceptible.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS. Labeled with horseradish peroxidase (HRP). No stabilizing proteins and no preservatives added.
