Kv4.2, a voltage-gated K+ (Kv) channel subunit, has been suggested to be the key component of the subthreshold A-type K+ currents (I(SA)s) recorded from the specific subcellular compartments of certain CNS neurons.
A-type channels, encoded by the pore-forming alpha-subunits of the Kv4.x family, are particularly important in regulating membrane excitability in the CNS and the heart.
Kv4.2 is a Shal-type potassium channel that passes an A-type current and is localized to dendrites and cell bodies in the hippocampus.
Deletion of the Kv4.2 gene and a loss of Kv4.2 protein resulted in a specific and near-complete elimination of A-type K+ currents from the apical dendrites of CA1 pyramidal neurons.
The absence of dendritic Kv4.2-encoded A-type K+ currents led to an increase of bAP amplitude and an increase of concurrent Ca2+ influx.
Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:200; Rat brain membranes using ECL
Immunohistochemistry: Rat brain sections
Note: Dilutions should be made using a carrier protein such as BSA (1-3%)
Optimal dilution determined by the researcher.
Control Antigen Included:K9018A: Kv4.2, Control Antigen (Shal1, RK5, Kcnd2, Voltage-gated Potassium Channel)Supplied as 40ug of lyophilized control antigen.
For negative control, preincubate 1ug of peptide with 1ug of antibody for one hour at RT.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 1% BSA, 0.025% sodium azide. Reconstitute with sterile ddH2O.
Specificity：Recognizes rat Kv4.2. Epitope Location: Intracellular, C-terminal part. The epitope specific for this antibody is not present in any other known proteins.
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Rt
Immunogen：Synthetic peptide corresponding to aa454-469, (C)SNQLQSSEDEPAFVSK of rat Kv4.2. Species Sequence Homology: mouse and human: 94%
