Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot (Rat brain membranes): 1:200 using ECL Immunohistochemistry: Rat brain sections Optimal dilutions to be determined by the researcher.
Control Antigen (Included):K9010A: Kv3.1b, Control Antigen (Kcnc1, Voltage-gated Potassium Channel)The stock solution of the antigen can be made up using 100ul of sterile deionized water.
For negative control, preincubate 1ug of peptide with 1ug of antibody for one hour at RT.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:50ul
Host:rabbit
Source Antibody:rat
Grade:Affinity Purified
Purity:Purified by affinity chromatography.
Form:Supplied as a lyophilized powder in PBS, pH 7.4, 1% BSA, 5% sucrose, 0.025% sodium azide. Reconsitute with 50ul sterile ddH2O.
Specificity:Recognizes full length rat Kv3.1b protein. Does not recognize any other potassium channel antigens. Species Crossreactivity: mouse, monkey and canine
Isotype:IgG
Calc Applications Abbrev:IHC IP WB
Calc Crossreactivity:Ca Mk Mo Rt
Immunogen:Synthetic peptide corresponding to aa567-585 (CKESP VIAKY MPTEA VRVT) of rat Kv3.1b.