The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors.
The functional diversity of these receptors is defined by the binding of acetylcholine to these receptors and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation.
Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system.
The muscarinic cholinergic receptor 2 is involved in mediation of bradycardia and a decrease in cardiac contractility.
Multiple alternatively spliced transcript variants have been described for this gene.
Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1:200 (Rat brain membranes)Immunohistochemistry: Rat brain sectionsOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, pH 7.4, 1% BSA, 5% sucrose, 0.05% sodium azide. Reconstitute with sterile ddH2O.
Specificity：Recognizes human Muscarinic Receptor M2. Species Crossreactivity: Rat and mouse. No cross reactivity with other muscarinic proteins tested so far.
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：GST fusion protein with a sequence of human m2 corresponding to aa225-356 (UniProt P08172). Species Sequence Homology: Chimpanzee, gorilla: identical; orangutan-130/132; porcine- 122/132; rat-118/132; mouse-117/132, canine-122/132.
