84-1027-33　Aquaporin 1 (AQP1, CHIP28) 100ug　A3000

特徴

• Water is a critical component of all living cells.
• Interestingly, tissue membranes show a great degree of water permeability.
• Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water.
• Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin".
• Aquaporin, are integral membrane proteins from a larger family of major intrinsic proteins (MIP) that form pores in the membrane of biological cells.
• Over the last decade, genes for several members of the aquaporin family have been cloned, expressed, and their distribution studied in many tissues.
• Aquaporin 1 (APQ1) is a 28kD integral membrane protein which was originally identified in red blood cells and renal proximal tubules.
• AQP1 is also expressed by the choroid plexus and various other tissues.
• Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:ELISA: 0.5-1.0ug/mlWestern Blot: 0.5-10ug/ml using Chemiluminescence technique.
• Reveal two bands, a lower 28kD band corresponding to unglycosylated form and an upper high molecular 35-60kD glycosylated component (Denker et al.
• 1988; Smith & Agre 1991).
• We recommend the use of 0.5-1.0% milk in all primary/secondary antibody-enzyme conjugate incubations in order to suppress non-specific bands.
• Immunohistochemistry: 2-10ug/ml on paraformaldehyde fixed tissue sections.
• Optimal dilution determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.