Applications: Suitable for use in ELISA, Immunocytochemistry and Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Crossreactivity Data:Using a conjugate Choline-GA-PC, antibody specificity was performed with an ELISA test by competion experiments with the following compounds.
CompoundCrossreactivity ratio (a)Choline-PC 1Phosphatidylcholine (free) >1:10,000Choline (free)>1:10,000Acetylcholine (free)>1:10,000
(a): Choline-PC concentration/unconjugated or conjugated analogs concentration at half displacement.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Grade：Purified
Purity：Purified
Form：Supplied as a lyophilized powder. Reconstitute with 100ul sterile ddH20.
Specificity：Recognizes protein conjugated Acetylcholine. See additional crossreactivity data.
Isotype：IgG
Calc Applications Abbrev：E FC IC
Immunogen：Synthetic choline conjugated to protein carrier (PC).
