84-1027-20　Survivin, CT (TIAP, API4) 100ug　S8500-04

特徴

• Apoptosis, or programmed cell death, is related to many diseases, such as cancer.
• Apoptosis is triggered by a variety of stimuli including members in the TNF family and prevented by the inhibitor of apoptosis (IAP) proteins.
• IAP proteins form a conserved gene family that binds to and inhibits cell death proteases.
• A novel IAP protein was recently identified and designated survivin, apoptosis inhibitor 4 (API4), and TIAP (1–3).
• Survivin/TIAP interacted with the processed form of caspase-3 and inhibited its proteolytic activity.
• Survivin/TIAP is predominantly expressed in tissues of embryos, transformed cell lines, and many human cancers and lymphomas (1,3).
• Applications:Suitable for use in Western Blotting.
• Other applications not tested.
• Recommended Dilution:Western Blotting: 0.5-1ug/ml.
• Optimal dilutions to be determined by the researcher.
• Positive Control: Mouse spleen tissue lysate
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
• Freeze at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.