84-1027-19　Histone H3, phosphorylated (Ser10) (Biotin) 100ug　H5110-14K

特徴

• Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes.
• Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4).
• The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
• This gene is intronless and encodes a member of the histone H3 family.
• Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element.
• This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
• Applications:Suitable for use in Western Blot and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilutions:Immunocytochemistry: 2-4ug/ml.
• Positive immunostaining of chromatin in HeLa cells undergoing mitosis.
• Cells were fixed with 4% paraformaldehyde and permeablized with 0.25% TritonX
• Western Blot: 0.5-2ug/ml detected phospho-Histone H3 in colcemid treated HeLa cell lysate.
• Optimal dilutions to be determined by researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.