The kinetophore is a macromolecular structure that is associated with the centromeres of chromosomes and is responsible for establishing and maintaining the connections with the microtubules of the mitotic spindle.
The proteins that reside within the chromosomal domain fall into two broad classes: those that associate with the centromere throughout the cell cycle and those that are found at the centromere in a cell cycle specific manner.
Members of the first class include CENP-A, CENP-B, and CENP-C.
The members of the second class include several ubiquitous cellular proteins such as calmodulin, tubulin, dynein and p34 cdc2 and also CENP-E and CENP-F.
CENP-F appears to be the earliest member to interact with the centromere-kinetochore complex.
Applications:Suitable for use in Western Blot.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 1:200-1:1000 detected CENP-A in RIPA lysates from HeLa cells.
20ug control histones, were resolved by electrophoresis, transferred to nitrocellulose and probed with C2615 (2ug/ml).
Proteins were visualized using n IgG (HRP) goat anti-rabbit secondary antibody and a chemiluminescence detection system
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide
Specificity：Recognizes human CENP-A, Mr17kD. Broad species cross-reactivity predicted due to sequence conservation.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa4-17, RRRSRKPEAPRRRS of human CENP-A, conjugated to KLH.
