84-1027-10　Nedd4, WW2 domain discontinued 200ul　N0577-15

特徴

• Neuronal precursor cell-expressed developmentally downregulated 4 (Nedd4) is an ~120kD E3 ubiquitin ligase.
• Nedd4 is involved in regulation of ubitiquin-targeted degradation of proteins.
• Nedd4 interacts with many different E2 enzymes, including human Ubc4, UbcH5B, UbcH5C, UbcH6 and UbcH7, suggesting it plays a role in multiple E2 ubiquitination pathways.
• Nedd4 contains a C2 domain and several WW domains, which recognize membrane surfaces and Pro-Tyr motifs, respectively.
• Nedd4 polyubiquitinates the epithelial sodium channel, and mutations that disrupt association between Nedd4 and the channel lead to Liddle’s syndrome, a hyperaldosteronism-like, hypertension disease.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications have not been tested.
• Recommended Dilutions:Western Blot: 1:10,000 detects Nedd4, WW2 domain on 10ug of mouse kidney cytosol.
• Mouse kidney cytosol lysate was resolved by electrophoresis, transferred to PVDF and probed with N0577-15.
• Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system
• Immunohistochemistry: Reported to detect Nedd4 in mouse kidney, lung and distal colon sections
• Optimal dilutions to be determined by the researcher.
• Recommended Secondary Antibodies:I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x RbI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x RbI1904-46Q: IgG, H&L (HRP) Pab Gt x Rb
• Positive Control:Kidney cytoplasmic extracts
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.