Two retinoblastoma-related proteins, p107 and pRb2/p130 are structurally and functionally similar to the product of the retinoblastoma gene (pRb/p105) Like pRb they bind transforming proteins of DNA tumor viruses through a particular region called the “pocket domain.”.
Both proteins play a fundamental role in growth control.
The highest percentage of undetectable levels and the tightest inverse correlation with the histological grading and with PCNA expression in the most aggressive tumor types were found for pRb2/p130, which may suggest an important role for this protein in the pathogenesis and progression of lung cancer.
Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation, and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilution:ELISA: 1:5000-1:20,000Western Blot: 1:500-1:2000Immunohistochemistry (Paraffiin embedded): 1:200-1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in PBS, pH7.2, with 0.01% sodium azide. No stabilizing proteins added.
Specificity：Recognizes a 130kD Rb2 protein from human tissue. Species crossreactivity: rat and mouse. No reaction was observed against other related tumor supressor proteins. Crossreactivity with Rb2 (p130) from other species may also occur.
Isotype：IgG
Calc Applications Abbrev：E IHC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Rb2 (p130) peptide corresponding to the C-terminus of the human protein (KLH).
