Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation, and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:2000-1:4000Western Blot: 1:250-1:1000Immunohistochemistry (paraffin embedded sections): 1:25-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：250ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in 125mM sodium borate, 75mM sodium chloride, 5mM EDTA, pH 8.0, 0.01% sodium azide.
Specificity：Recognizes human laminin. Typically less than 1% crossreactivity against other extracellular matrix proteins was detected by ELISA against purified standards. Recognizes most mammalian Laminins and has negligible crossreactivity with Type I, II, III, IV, V or VI Collagens or Fibronectin. Non-specific crossreaction of anti-Laminin antibodies with other human serum proteins or non-Laminin extracellular matrix proteins is negligible.
Isotype：IgG
Calc Applications Abbrev：E IHC IP WB
Calc Crossreactivity：Hu Ma
Immunogen：Synthetic peptide corresponding to regions of adherence on laminin.
