84-1026-91　Cyclin D1 100ul　C8454-04

特徴

• Cyclin D1 gene is identical with the BCL-1 and the PRAD-1 oncogene.
• It is part of the chromosome 11q13 amplicon which is amplified in several common tumor types like breast, lung, esophagus, urinary bladder and head and neck carcinoma.
• Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms.
• About 50-70% of mantle cell lymphomas (MCL) contain a t (11; 14)(q13; q32) translocation resulting in over-expression of cyclin D1.
• Cyclins were originally identified as proteins whose levels fluctuated during the cell cycle.
• Subsequent studies have shown the cyclins control entry into different phases of the cycle cell by regulating the activity of cyclin-dependent kinases (CDKs).
• Mitotic or G2 cyclins regulate entry into M phase.
• G1 cyclins regulate entry into S phase.
• Cyclin D1, a G1 cyclin, is essential for control at the G1/S transition (START in yeast).
• It regulates the activity of CDK4 and CDK6 protein kinases.
• Translocation of the gene encoding cyclin D1, the bcl-1 oncogene, is associated with human B-lymphocytic malignancy, suggesting that alteration of cell cycle progression may be oncogenic.
• Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:2000-1:10,000Western Blot: 1:500-1:1000Immunoprecipitation: 1:100Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.