The protein encoded by this gene is a regulatory protein involved in mitosis.
The gene product forms a complex with p34 (cdc2) to form the maturation-promoting factor (MPF).
Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript that isexpressed predominantly during G2/M phase.
The different transcripts result from the use of alternate transcription initiation sites.
Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:2000-1:10,000Western Blot: 1:500-1:1000Immunoprecipitation: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ul
Host:rabbit
Source Antibody:human
Grade:Serum
Purity:Purified by delipidation and defibrination.
Form:Supplied as a liquid, 0.01% sodium azide. No stabilizing proteins added.
Specificity:Recognizes human Cyclin B1 between 55-62kD. No reaction was observed against other related cyclins. Species Crossreactivity: rat and mouse.
Isotype:IgG
Calc Applications Abbrev:E IHC IP WB
Calc Crossreactivity:Hu Mo Rt
Immunogen:Full length GST fusion protein corresponding to human Cyclin B1.