Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:5,000-1:50,000Western Blot: 1:1000-1:10,000Immunohistochemistry (Frozen sections): 1:50-1:200 Immunoprecipitation: 1:100Optimal dilutions to be determined by the researcher.
Note: Some class-specific anti-collagens may be specific for non-denatuted three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.
Greatly diminished reactivity and selectivity of class specific anti-collagen antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in BBS, pH 8.0, 0.005M EDTA, 0.01% sodium azide.
Specificity：Recognizes human Collagen Type IV. Typically less than 1% crossreactivity against other types of collagens was detected by ELISA against purified standards. Reacts with most mammalian Type IV collagens and has negligible crossreactivity with Type I, II, III, V or VI collagens. Non-specific crossreactivity with other human serum proteins or non-collagen extracellular matrix proteins is negligible. Species Crossreactivity: bovine
Isotype：IgG
Calc Applications Abbrev：E IHC IP WB
Calc Crossreactivity：Hu Ma
Immunogen：Collagen Type IV from human and bovine placenta
