Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:100ug
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in ,0.125.M sodium borate, 0.075M sodium chloride, 10mg/ml BSA (IgG and Protease free), 0.005M EDTA 0.09% sodium azide. Biotin conjugated
Specificity:Recognizes human Collagen Type II. Typically less than 1% crossreactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. Recognizes most mammalian Type II collagens and has negligible crossreactivity with Type I, III, IV, V and VI collagens. Non-specific crossreaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Isotype:IgG
Calc Applications Abbrev:E IHC IP WB
Calc Crossreactivity:Hu
Immunogen:Collagen Type II from adult human knee cartilage and bovine nasal cartilage.