Western Blot (Non-denaturing, non-dissociating): 1:2500-1:5000
Immunohistochemistry (frozen tissue sections): 1:25-1:100Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.125M sodium borate, 0.075M sodium chloride, 0.005M EDTA, pH 8.0, 0.01% sodium azide before the addition of glycerol to 40%. No stabilizing proteins added.
Specificity：Recognizes Type II collagens. Species crossreactivity: most mammals. Crossreactivity with Type I, III, IV, V or VI collagens is negligible. Non-specific crossreaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Isotype：IgG
Calc Applications Abbrev：E IHC IP WB
Calc Crossreactivity：Hu
Immunogen：Collagen Type II from adult human knee cartilage and bovine nasal cartilage. Collagen Type II was extensively purified by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein.
