Applications:Suitable for use in Western Blot, ELISA, Immunohistochemistry and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:3000-1:6000ELISA: 1:3000-1:6000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Biotin/Protein Ratio:10-20 BAC molecules per rabbit IgG molecule
Storage and Stability:Lyophilized powder may be stored at 4°C Stable for 12 months.
Reconstitute with sterile 40-50% glycerol, ddH2O.
Aliquot and store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 0.125M sodium borate, 0.075M sodium chloride, 0.005M EDTA, pH 8.0, 10mg/ml BSA, 0.01% sodium azide. Labeled with Biotin. Reconstitute with100ul sterile 40-50% glycerol, ddH2O.
Specificity：Recognizes Collagen Type I. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. Recognizes most mammalian Type I collagens and has negligible crossreactivity with Type II, III, IV, V and VI collagens detected by ELISA against purified standards.. Non-specific crossreaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Isotype：IgG
Calc Applications Abbrev：E IHC IP WB
Calc Crossreactivity：Bo Hu
Immunogen：Collagen Type I from human and bovine placenta.
