84-1026-73　Collagen Type I 100ug　C7510-11C

特徴

• Collagens are highly conserved throughout evolution and are characterized by a n uninterrupted “Glycine-X-Y” triplet repeat that is a necessary part of the triple helical structure.
• It is often extremely difficult to generate antibodies with specificities to collagens.
• The development of type specific antibodies is dependent on non-denatured three-dimentional epitopes.
• Collagens from human and bovine placenta and cartilage were extensively purified by limited pepsin digestion and selective salt precipitation.
• This preparation resulted in a native conformation of the protein.
• Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to product “type” specific antibodies.
• Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducting conditions of SDS-PAGE and Immunoblot are used.
• Applications:Suitable for use in ELISA, Immunoprecipitation, native PAGE, Immunohistochemistry and Western Blot Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:10,000ELISA: 1:5000-1:50,000Immunohistochemistry: 1:50-1:200Immunoprecipitation: 1:100Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.