The positive transcription elongation factor b (P-TEFb) is identified as cdk9 paired with cyclin T1.
The cyclin T1/cdk9 (PITALRE) complex phosphorylates the carboxy terminal domain of RNA Polymerase II in vitro and associate with HIV-1 generated Tat in HIV-1 infected T-cells.
Tat stimulates human HIV-1 viral transcription elongation.
This suggests that cyclin T1/cdk9 (PITALRE) is one of the HIV-1 required host cellular cofactors generated during T-cell activation.
Cyclin T1/cdk9 (PITALRE) is shown to interact with Tat to restore Tat activation in HeLa nuclear extracts depleted of P-TEFb.
The cdk9 (PITALRE) activity and cyclin T1 are essential for activation of transcription when tethered to the heterologous Rev response element RNA via the regulator of expression of virion Rev.
Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000-1:50,000Western Blot: 1:500-1:3000Immunohistochemistry (Paraffin): 1:200-1:1000 Immunoprecipitation: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.
Specificity：Recognizes human cdk9 (PITALRE) protein at 43kD. No reaction was observed against other related cyclin dependent kinases. Species crossreactivity: rat and mouse. The murine cDNA is shown to be 98% identical with human.
Isotype：IgG
Calc Applications Abbrev：E IHC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Multiple synthetic peptides corresponding to C-terminal and N-terminal domains of the protein coded by the human gene cdk9 (PITALRE)
