Applications:Suitable for use in ELISA, Immunoprecipitation, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 1:5000-1:10,000 Immunoprecipitation: 1:100Western Blot: 1:5000-1:10,000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:500ug
Host:goat
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography
Form:Supplied as a liquid in 0.125M sodium borate, 0.075M sodium chloride, 0.005M EDTA, pH 8.0, 0.01% sodium azide. No stabilizing proteins added.
Specificity:Recognizes human apolipoprotein J and has negligible crossreactivity with Type A-I, A-II, B, C-I, C-II, C-III and E apolipoproteins. Typically less than 1% crossreactivity against other types of apolipoprotein was detected by ELISA against purified standards. Non-specific crossreaction of anti-apolipoprotein antibodies with other human serum proteins is negligible.
Isotype:IgG
Calc Applications Abbrev:E IHC IP WB
Calc Crossreactivity:Hu
Immunogen:Apolipoprotein Type J isolated from human plasma by density gradient centrifugation followed by HPLC purification.