Perforin, originally cloned from mouse cytotoxic T-lymphocyte (CTL), is a 555-amino acid protein with a 21-amino acid signal peptide and is a potent cytolytic pore-forming protein.
It is a specific marker of functionally active cytotoxic T-lymphocytes (CTLs) and natural killer (NK) cells.
Fas ligand and perforin are the two key effector mechanisms in T cell-mediated cytotoxicity.
These molecules mediate cytolysis of target cells by membrane damage and apoptosis, an important mechanism in the immune defense against tumors and virus infection.
Perforin expression is constitutive on NK cells, but increases in resting XD8+ cytotoxic cells upon activation.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：200ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a lyophilized powder, 0.09% sodium azide. Reconstitute with PBS.
Specificity：Recognizes rat perforin. Species Crossreactivity: Mouse.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Mo Rt
Immunogen：Full length recombinant protein corresponding to rat perforin, expressed in E.coli.
