Protein G is a small globular cell surface protein produced by Streptococcus sp.
It is composed of two or three nearly identical domains of 55 amino acids each.
It binds to Fc fragment (constant region) of Immunoglobulin G with high affinity.
Protein G can be used to detect, quantify and purify IgG antibodies and antibody/antigen complexes.
Protein G binds to all IgG subclasses from human, mouse and rat species.
It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep and horse.
Applications:Suitable for use in Immunoblot (Western or Dot Blot), ELISA, immunoperoxidase electron microscopy and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:20,000 to 1:100,000.
Assayed against 1ug of P9101-20 in a standard capture ELISA using ABTS (A0030) as a substrate for 30 min at RT
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：500ug
Host：rabbit
Grade：Highly Purified
Purity：Purified from monospecific antiserum by delipidation, fractionation and ion exchange chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, BSA (IgG and protease free), 0.01% gentamicin sulfate, 40% glycerol. Conjugated to HRP.
Specificity：Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-rabbit serum as well as purified and partially purified Protein G (Streptococcus sp.)
