Protein A is a highly stable surface receptor produced by Staphylococcus aureus, which is capable of binding the Fc portion of immunoglobulins, especially IgGs, from a large number of species (Boyle and Reis, 1987).
Each protein A molecule can bind 2 molecules of IgG, allowing the formation of a precipitate (Sjöholm, 1975).
Applications:Suitable for use in FLISA, Western Blot and Immunofluorescence.
Other applications have not been tested.
Recommended Dilution:Immunofluorescence: 1:500-1:2500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
Aliquots are stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Texas Red conjugates are sensitive to light.
仕様
Size：500ug
Host：goat
Grade：Highly Purified
Purity：Purified from monospecific antiserum by delipidation, fractionation and ion exchange chromatography.
Form：Supplied as a liquid in PBS, 10mg/ml BSA (Immunoglobulin and Protease free), pH 7.2, 0.01% sodium azide.
Specificity：Recognizes Protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Protein A [Staphylococcus aureus], anti-goat IgG and anti-goat serum.
