Protein A is a highly stable surface receptor produced by Staphylococcus aureus, which is capable of binding the Fc portion of immunoglobulins, especially IgGs, from a large number of species (Boyle and Reis, 1987).
Each protein A molecule can bind 2 molecules of IgG, allowing the formation of a precipitate (Sjöholm, 1975).
Applications:Suitable for ELISA, Western Blot, Immunoprecipitation, Immunodiffusion, Conjugation and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:ELISA: 1:2000-1:100,000Western Blot: 1:2000-1:10,000Immunohistochemistry: 1:1000-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10mg
Host：goat
Grade：Highly Purified
Purity：Purified by delipidation, salt fractionation, and ion exchange chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide, 40% glycerol. No stabilizing proteins added.
Specificity：Recognizes Protein A. Assay by IEP resulted in a single precipitin arc against purified and partially purified Protein A (S. aureus) and anti-goat serum.
