Protein A is a highly stable surface receptor produced by Staphylococcus aureus, which is capable of binding the Fc portion of immunoglobulins, especially IgGs, from a large number of species (Boyle and Reis, 1987).
Each protein A molecule can bind 2 molecules of IgG, allowing the formation of a precipitate (Sjöholm, 1975).
Applications:Suitable for use in Immunoblotting (Western and Dot Blot), ELISA and Immunoprecipitation.
Other applications have not been tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：2ml
Host：goat
Grade：Serum
Purity：Delipidated and defibrinated antiserum.
Form：Supplied as a powder lyophilized from a solution containing PBS, pH 7.4, and 0.01 % sodium azide. Reconstitute to 2ml with ddH2O.
Specificity：Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Protein A (S. aureus).
