Applications:Suitable for use in Immunoblot (Western or Dot blot), ELISA and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilution:ELISA: 1:500-2500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:500ug
Host:sheep
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in 50mM Tris-HCl, pH 8.0, 150mM sodium chloride, 1mM magnesium chloride, 0.1mM zinc chloride, 50% glycerol, 10mg/ml BSA, 0.01% sodium azide.
Specificity:Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine), anti-sheep serum and cyanine conjugated IgG. Reactive with Cy2, Cy3 and Cy5.