Applications:Suitable for use in ELISA, Immunohistochemistry and and Western Blot.
Other applications have not been tested.
Recommended Dilution:ELISA: 1:10,000 - 1: 50,000Immunohistochemistry: 1:1,000 -1: 5,000Western Blot: 1:2,000 - 1:10,000Optimal dilutions to be determined by the researcher.
Biotin/Protein Ratio:10-20 BAC molecules per goat IgG molecule
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:1mg
Host:goat
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a powder lyophilized from a solution containing PBS, pH 7.2, 10mg/ml BSA (IgG and Protease Free) and 0.01% sodium azide. Labeled with Biotin. Reconstitute with 1ml sterile ddH2O.
Specificity:Assay by immunoelectrophoresis resulted in a single precipitin arc against anti biotin, anti goat serum and fluorescein conjugated IgG.