Applications:Suitable for Western Blot, ELISA and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilution:ELISA: 1:500 to 1:2500Optimal dilutions to be determined by the researcher.
Storage and Stability:Store at 4° C before opening.
DO NOT FREEZE.
This product is stable at 4° C as an undiluted liquid.
Dilute only prior to immediate use.
Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
仕様
Size:1mg
Host:goat
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in 0.05M Tris chloride, 0.15M sodium chloride, 0.001M Magnesium Chloride, 0.001M zinc chloride, pH 8.0, 10mg/ml BSA, 0.01% sodium azide and 50% glycerol. Labeled with alkaline phosphatase.
Specificity:Recognizes Biotin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-AP, anti-goat serum and biotinylated IgG. No reaction was observed against anti-pepsin or anti-goat IgG F(c).
Isotype:F(ab’)2
Calc Applications Abbrev:E IHC WB
Immunogen:Biotin conjugated to Keyhole Limpet Hemocyanin (KLH).